Short-Term Storage of Rooster Ejaculates: Sperm Quality and Bacterial Profile Differences in Selected Commercial Extenders

Bacterial contamination of semen has become an important contributor to the reduced shelf life insemination doses in poultry industry, which is why antibiotics (ATBs) are component extenders. Due a global rise antimicrobial resistance, aim this study was assess efficiency selected commercially available extenders prevent possible bacterial rooster ejaculates. Two free from or containing 31.2 µg/mL kanamycin (KAN) were used process samples 63 healthy Lohmann Brown roosters. Phosphate-buffered saline without ATBs as control. The extended stored at 4 °C for 24 h. Sperm motility, viability, mitochondrial activity, DNA integrity and oxidative profile each sample assessed following 2 h storage. Furthermore, selective media quantify load specific species identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. results indicate that enriched KAN ensured significantly higher preservation sperm quality comparison their KAN-free counterparts. decreased diluents supplemented (p ≤ 0.001); however, alone not effective enough eradicate all bacteria since several Escherichia coli, Enterococcus faecalis, faecium Micrococcus luteus retrieved KAN-supplemented commercial As such, we may suggest more focus should be devoted selection optimal combination dose extenders, accompanied by frequent bacteriological screening native well semen.